Regulation of Regenerative Periodontal Healing by NAMPT
Figure 5
Effect of EMD on the nuclear translocation of SMAD1/5/8 in the presence and absence of NAMPT (100 ng/mL) at 60 min, as determined by immunofluorescence (a). Untreated cells served as control. Stimulation of SMAD1/5/8 phosphorylation by EMD over 60 min, as analyzed by immunoblotting (b). Effect of EMD on SMAD1/5/8 phosphorylation in the presence and absence of NAMPT (100 ng/mL) at 60 min, as examined by immunoblotting (c). All experiments were performed in triplicate and repeated at least twice. Images and blots from one representative donor are shown.
