Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
Figure 3
Innate sensing of PAMPs in human MDMs via TLR4 and TLR7/8. MDMs were produced during a 7-day period through stimulation by four differentiation protocols. The different MDM types were stimulated with either LPS (100 ng/ml), R848 (5 μg/mL), or left untreated (UT) for 6 hours before isolation of supernatants. Levels of CCL5 and CXCL10 were measured by ELISA. Data shown are mean values ± SEM from 10 donors from 3 separate experiments.
