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Mediators of Inflammation
Volume 2013, Article ID 231351, 13 pages
http://dx.doi.org/10.1155/2013/231351
Research Article

Lipoxin A4 Preconditioning and Postconditioning Protect Myocardial Ischemia/Reperfusion Injury in Rats

1Department of Cardiovascular and Thoracic Surgery, The 2nd Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, 109 College Western Road, Wenzhou 325027, China
2Department of Cardiovascular Medicine, The 2nd Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, 109 College Western Road, Wenzhou 325027, China

Received 10 April 2013; Revised 15 June 2013; Accepted 16 June 2013

Academic Editor: Fulvio D'Acquisto

Copyright © 2013 Qifeng Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aims to investigate the pre- and postconditioning effects of lipoxin A4 (LXA4) on myocardial damage caused by ischemia/reperfusion (I/R) injury. Seventy-two rats were divided into 6 groups: sham groups (C1 and C2), I/R groups (I/R1 and I/R2), and I/R plus LXA4 preconditioning and postconditioning groups (LX1 and LX2). The serum levels of IL-1β, IL-6, IL-8, IL-10, TNF-α, and cardiac troponin I (cTnI) were measured. The content and the activity of Na+-K+-ATPase as well as the superoxide dismutase (SOD), and malondialdehyde (MDA) levels were determined. Along with the examination of myocardium ultrastructure and ventricular arrhythmia scores (VAS), connexin 43 (Cx43) expression were also detected. Lower levels of IL-1β, IL-6, IL-8, TNF-α, cTnI, MDA content, and VAS and higher levels of IL-10, SOD activity, Na+-K+-ATPase content and activity, and Cx43 expression appeared in LX groups than I/R groups. Besides, H&E staining, TEM examination as well as analysis of gene, and protein confirmed that LXA4 preconditioning was more effective than postconditioning in preventing arrhythmogenesis via the upregulation of Cx43. That is, LXA4 postconditioning had better protective effect on Na+-K+-ATPase and myocardial ultrastructure.