IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo

Figure 1

IRF5 levels and cytokine response of in vitro differentiated macrophages. BMDMs were differentiated with GM-CSF (20 ng/mL) or M-CSF (100 ng/mL) for eight days. All cells were challenged with LPS for the indicated time periods. (a) Transcript levels were measured with real-time PCR. Error bars represent the standard error for 𝑛=6. Protein levels of IRF5 and β-actin were determined by western blot. Experiment is representative for three independent experiments. (b) and (c) At each time point RNA (top panel) and supernatants (bottom panel) were collected. Error bars represent the standard error for 𝑛=5. Statistical analysis was performed by 2-way ANOVA and Bonferroni’s multiple comparison. *𝑃≤0.05; **𝑃≤0.01; ***𝑃≤0.001; ****𝑃≤0.0001.

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