Mediators of Inflammation / 2013 / Article / Fig 1

Research Article

Dose-Dependent Anti-Inflammatory and Neuroprotective Effects of an ανβ3 Integrin-Binding Peptide

Figure 1

The appearance of lesion site in each group observed with MRI scanning was in accord with the severity of inflammatory cells infiltration showed by H&E staining: bar = 100 μm. (a) Normal group, T2W image of brain, without detectable changes occuring in the white matter. (b) The same animal as in (a). Histological staining. (c) Vehicle control rats at week 2 after immunization, T2W image. An arrow indicates clear hyperintensity area in the white matter. (d) The same animal as in (c). H&E staining, an arrow shows abundant cellular infiltrates under meninges. (e) 0.5 mg/day C16 treated EAE rats at week 2 after immunization. T2W image, an arrow showed a focal area with increased intensity. (f) The same animal as in (e). H&E staining. The arrow denotes “perivascular cuffing” of inflammatory cells. (g) C16 late treated EAE rats at week 2 after immunization. T2W image, an increased intensity lesion area was defined by arrow. (h) The same animal as in (g). H&E staining. (i) At week 8 after immunization. T2W image, arrow denotes an increased intensity lesion area, the same animal as in (j). H&E staining showed less severe inflammatory cell infiltration, and a lesion area was found at week 8 smaller than that at week 2 after immunization. (k) 2 mg/day C16 treated EAE rats at week 8 after immunization, T2W image. (l) The same animal as in (k). H&E staining. ((m)–(p)) Diffuse infiltration of inflammatory cells was observed in the spinal cord of the vehicle control rats, and attenuated in C16 treated EAE rats at week 2 after immunization. H&E staining, traverse section through the lumbar spinal cord; bar = 100 μm. Normal rats group (m), vehicle control rats (n), 2 mg /day C16 treated EAE rats (o), and C16 late treated EAE rats (p). (q) Medium and high dose C16 treatment reduced inflammatory cells showed by inflammation score. versus normal rats; versus vehicle control rats at week 2 postimmunization group; versus vehicle control rats; versus 0.5 mg/day C16 treated EAE rats at week 8 after immunization. (r) C16 late treatment also inhibited inflammatory cells infiltration to a certain extent showed by inflammation score. versus normal rats; versus vehicle control rats at week 2 after immunization; versus C16 treated EAE rats at week 2 postimmunization group; versus vehicle control rats at week 8 after immunization.
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