The Endothelial Tyrosine Phosphatase SHP-1 Plays an Important Role for Vascular Haemostasis in TNFα-Induced Inflammation In Vivo
Figure 3
Inhibition of SHP-1 increases TNFα-induced upregulation of p-selectin and vWF in endothelial cells in vitro. (a) Incubation of endothelial cells with the pharmacological SHP-1 inhibitor sodium stibogluconate effectively reduces SHP-1 activity as measured by pNPP-dephosphorylation assay. (b) Endothelial p-selectin was not changed by sodium stibogluconate under basal conditions but, significantly upregulated in TNFα-induced endothelial inflammation. (c) vWF was already upregulated by sodium stibogluconate under basal conditions to a similar level as observed in TNFα-induced endothelial inflammation. SG: sodium stibogluconate. *Significantly different at (HUVEC, ).
