Pharmacological Inhibition of p38 Mitogen-Activated Protein Kinases Affects KC/CXCL1-Induced Intraluminal Crawling, Transendothelial Migration, and Chemotaxis of Neutrophils In Vivo
Figure 3
Effect of SB203580 on parameters of KC-induced intraluminal crawling of neutrophils. (a) The percentage of adherent cells that crawl in the luminal surface of the endothelium upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). * indicates significant difference () from the control without SB203580 (t-test). (b) The total distance (μm) traversed by a crawling neutrophil (averaged from >40 cells) in the luminal surface of the endothelium upon stimulation with KC in the absence (Control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). (c) The duration (min) of neutrophil crawling in the luminal surface of the endothelium upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). (d) The velocity (μm/min) of neutrophil crawling in the luminal surface of the endothelium upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). (e) The percentage of crawling neutrophils that crawl along the blood flow in the luminal surface of the endothelium upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). (f) The directional crawling index (CI) of neutrophil in the luminal surface of the endothelium upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM ().
