Pharmacological Inhibition of p38 Mitogen-Activated Protein Kinases Affects KC/CXCL1-Induced Intraluminal Crawling, Transendothelial Migration, and Chemotaxis of Neutrophils In Vivo
Figure 4
Effect of SB203580 on parameters of KC-induced neutrophil transmigration. (a) The percentage of crawling cells that transmigrate across the endothelium upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). ** indicates significant difference () from the control without SB203580 (t-test). (b) The duration (min) of neutrophil transmigration across the endothelium upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). * indicates significant difference () from the control without SB203580 (t-test). (c) Detachment time (min) of transmigrated neutrophils from the venule upon stimulation with KC in the absence (control, white) and in the presence (black) of p38 MAPK inhibitor SB203580 (100 nM) 30 min prior to and 60 min following the placement of KC-containing gel. Data are means ± SEM (). The statistical difference between the two groups was found to be (not significant; t-test).
