Plasmacytoid Dendritic Cell Response to CpG ODN Correlates with CXCL16 Expression and Is Inhibited by ox-LDL
Figure 3
CXCL16/CXCR6 interaction reduces the threshold of activation in pDCs responding to D-ODN. (a) pDCs were enriched from human PBMCs using the BDCA-4 isolation kit as recommended by the manufacturer. Purity of cells was established by flow cytometry following staining with pDC associated cell-surface markers. (b) Enriched pDCs (100,000 cells/well) were incubated with CXCR6 or CCR5 expressing Jurkat T cells (1 : 1 ratio) in 96-well U-bottom plates. Cocultures were then stimulated with a suboptimal concentration of D-ODN (0.75 μg/mL). pDC stimulated with optimal concentration of D-ODN (10 μg/mL) served as a positive control. Cytokine production was assessed from culture supernatants 24 h later using ELISA. Average response of 3 different pDC preparations is shown (mean ± S.D).
