Plasmacytoid Dendritic Cell Response to CpG ODN Correlates with CXCL16 Expression and Is Inhibited by ox-LDL
Figure 4
Oxidized LDL and recombinant IFNγ/TNFα modify cytokine production induced by D-ODN. (a)Enriched pDCs (100,000 cells/well) were preincubated with LDL or OxLDL (10 μg/mL each) and then stimulated with 3 μM of D-ODN. Cytokine production was assessed from culture supernatants 24 h later using ELISA. Average response of 3 different pDC preparations is shown (mean ± S.D). (b)Enriched pDCs were preincubated in the absence or presence of GM6001 (50 μM), recIFNγ/TNFα (20 ng/mL each), or PMA/ionomycin (250 pg/mL/100 pg/mL) for 30 min at 37°C. Cytokine production was assessed from culture supernatants 24 h later using ELISA. Average response of 3 different pDC preparations is shown (mean ± S.D). (c)PBMC (4 × 106/mL) were preincubated in the absence or presence of 1 μM Control ODN, 1 μM K-ODN, or 3 μM D-ODN for 48 h. Cells were then fixed and stained for CXCL16 expression. MFI of CXCL16 stained cells ± S.D of 3 different donors is shown.
