Research Article
Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum
Table 1
DNA sequences of the sense and antisense primers for RT-PCR analysis and cycler conditions.
| | Gene | Accession number (GeneBank) | Primer sequences | Product size (bp) | Annealing temperature (°C) | Number of cycles |
| | Preproinsulin | XM_003508080.1 | 5′-CTTTTGTCAAACAGCACCTTTGTG-3′ (sense)
5′-TCAGTTGCAGTAGTTCTCCAGTTGG-3′ (antisense) | 263 | 48 | 25 | | PDX-1 | XM_003496658.1 | 5′-CAGCTCCCTTTCCCGTGG A-3′ (sense)
5′-GAGCATCACTGCCAGCTCCA-3′ (antisense) | 204 | 55 | 28 | | Nrf2 | XM_003498316.1 |
5′-CTCACTGGATAAAGAAGTC-3′ (sense) 5′-CCGCCCAGAAGTTCAGAGA-3′ (antisense) | 240 | 60 | 40 | | β-Actin | NM_031144.2 | 5′-AAGAGAAGCTGTGCTATGTTGC-3′ (sense)
5′-CCTTGATCTTCATGGTGCTAGG-3′ (antisense) | 324 | 48 | 25 |
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PDX-1, Pancreatic and duodenal homeobox 1; Nrf2, Nuclear factor (erythroid-derived 2)-like 2.
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