Oncostatin M and TLR-4 Ligand Synergize to Induce MCP-1, IL-6, and VEGF in Human Aortic Adventitial Fibroblasts and Smooth Muscle Cells
Figure 4
Cell signalling pathways activated in HAoAFs after 20-minute stimulation. HAoAFs were stimulated for 20 minutes with media (control), LPS (10 ng/mL), OSM (either 0.5 or 5 ng/mL), or a combination of LPS (10 ng/mL) and OSM (either 0.5 or 5 ng/mL), and total cell lysates were prepared. Immunoblots were probed with the antibodies indicated (a) and densitometry was used to quantitatively assess signal strength, corrected to total protein for each probe, and expressed normalized to control (unstimulated) levels.
