Oncostatin M and TLR-4 Ligand Synergize to Induce MCP-1, IL-6, and VEGF in Human Aortic Adventitial Fibroblasts and Smooth Muscle Cells
Figure 7
Regulation of STAT-3 and MCP-1 by OSM verses gp130 cytokines. HAoAF (upper panel in (a)) and HAoSMC (lower panel in (a)) were stimulated for 20 minutes with media (control, C) or OSM, LIF, IL-11, IL-31, or IL-6 (all at 5 ng/mL) with or without LPS (10 ng/mL) as indicated. Immunoblots were probed for pSTAT3 and total STAT3 as a loading control. In (b), HAoAFs were stimulated for 6 hours with media alone (control, C) or OSM, LIF, IL-11, IL-31, or IL-6 (all at 5 ng/mL) with or without LPS (10 ng/mL) as indicated. Steady state levels of MCP-1mRNA were measured by qRT-PCR and expressed relatively to housekeeping gene 18S.
