Sildenafil (Viagra) Protective Effects on Neuroinflammation: The Role of iNOS/NO System in an Inflammatory Demyelination Model

<table>Western blotting for GSTpi. (a) Immunoblot of iNOS<sup>−/−</sup> control (Cont), cuprizone (CPZ), and cuprizone plus sildenafil (CPZ + Sild) groups. (b) Graph represents quantification and statistical analysis. iNOS<sup>−/−</sup> control showed basal expression of GSTpi. After CPZ, GSTpi decreased significantly, compared to iNOS<sup>−/−</sup> cont. CPZ + Sild treatment increased GSTpi expression in relation to the CPZ group, but the levels of this protein remained significantly decreased compared to control. The experiment was performed in triplicate. ***<svg height="11.375" id="M29" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 66.875 11.375" width="66.875" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> compared to control; <sup>###</sup><svg height="11.375" id="M30" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 66.875 11.375" width="66.875" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> compared to CPZ.</table>

Mediators of Inflammation

fig7

Figure 7

Figure 7: Sildenafil (Viagra) Protective Effects on Neuroinflammation: The Role of iNOS/NO System in an Inflammatory Demyelination Model 