Figure 7: Western blotting for GSTpi. (a) Immunoblot of iNOS−/− control (Cont), cuprizone (CPZ), and cuprizone plus sildenafil (CPZ + Sild) groups. (b) Graph represents quantification and statistical analysis. iNOS−/− control showed basal expression of GSTpi. After CPZ, GSTpi decreased significantly, compared to iNOS−/− cont. CPZ + Sild treatment increased GSTpi expression in relation to the CPZ group, but the levels of this protein remained significantly decreased compared to control. The experiment was performed in triplicate. *** compared to control; ### compared to CPZ.