Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδ/c-Src/EGFR/PI3K/Akt/AP-1 Signaling Pathway in Human Chondrocytes
Figure 2
Role of PKCδ in thrombin-induced MMP-13 production. ((a) and (b)) qPCR and ELISA analyses of MMP-13 expression in chondrocytes, which were pretreated with rottlerin (3 μM) for 30 min or transfected with siRNA against PKCδ for 24 h and then stimulated with thrombin for 24 h. ((c) and (d)) Western blotting analysis of phosphorylation and kinase activity of PKCδ in cells incubated with thrombin for the indicated time intervals. (e) Kinase activity of PKCδ in chondrocytes, which were transfected with siRNA against PAR1 or PAR3 for 24 h and stimulated with thrombin for 60 min. * as compared with basal levels. # as compared with the levels in the thrombin-treated group.
