Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδ/c-Src/EGFR/PI3K/Akt/AP-1 Signaling Pathway in Human Chondrocytes

Figure 3

Activation of c-Src in thrombin-mediated MMP-13 expression in chondrocytes. ((a) and (b)) qPCR and ELISA analyses of MMP-13 expression in chondrocytes that were pretreated for 30 min with PP2 (3 μM) or transfected with c-Src siRNA for 24 h and then stimulated with thrombin for 24 h. ((c) and (d)) Western blotting analysis of phosphorylation and kinase activity of c-Src in cells incubated with thrombin for the indicated time intervals. (e) Kinase activity of c-Src in chondrocytes pretreated for 30 min with rottlerin and stimulated with thrombin for 60 min. *𝑃<0.05 as compared with basal levels. ^#𝑃<0.05 as compared with the levels in the thrombin-treated group.

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