Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone
Surface characteristics and direct proof of EPC and MSC on Tutoplast by SEM. (a) shows a Tutoplast granule without cells, (b) in a higher magnification to demonstrate the fibrillar structure. (c), (d), and (e) show Tutoplast sown with EPC (c), with MSC (d), and with both cell types in combination (e). Early EPC appeared rather spindle shaped and elongated whereas the MSC demonstrated a more flattened and out-spread phenotype. Scanning electron microscopy was performed as described in the Materials and Methods Section. Bold type arrows indicate MSC, and spotted arrows indicate EPC. The scale bar indicates 3 μm (b), 60 μm (c, d), and 100 μm (a, e), respectively.
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