Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone
Expression of endothelial and osteogenic marker genes after 10 days on Tutoplast. Results of the real-time RT-PCR for vWF in EPC (a), VEGF in EPC and MSC (b), collagen-1 (c), alkaline phosphatase (ALP) (d), osteocalcin (BGLAP) (d), and the transcription factor cbfa-1 (RUNX) (e) in MSC are shown. Messenger RNA was isolated from either MSC, EPC, or MSC and EPC cultured on Tutoplast for 10 days. Reference RNA derived from EPC and MSC cultured under normal conditions was analyzed as well. Five MSC lines, respectively, five EPC lines were tested. Experiments were performed in duplicate. Gene expression was determined by the comparative ΔCt method as described in Section 2. Data are expressed as mean SEM of fold change to GAPDH gene expression. *: ; $: coculture (osteogenic conditions) versus MSC (osteogenic conditions).
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