Anti-Inflammatory Cytokine Interleukin-4 Inhibits Inducible Nitric Oxide Synthase Gene Expression in the Mouse Macrophage Cell Line RAW264.7 through the Repression of Octamer-Dependent Transcription
Figure 1
IL-4 inhibits IFNγ- and/or LPS-induced NO production and Nos2 mRNA expression in RAW264.7 cells. (a) RAW264.7 cells were treated with medium alone (untreated, UT) or IL-4 (10 ng/mL) for 30 min prior to stimulation with IFNγ (10 ng/mL) and/or LPS (100 ng/mL). The culture supernatants were harvested and assessed for NO production by the Griess assay. The protein concentrations of the residual cells in the cultures were also determined. Each column and bar represents the mean ± SEM of three independent experiments. The asterisks denote a statistically significant difference compared to the cultures with IL-4, (*; Student’s test). (b) RAW264.7 cells were treated with medium alone or IL-4 (10 ng/mL) for 30 min prior to stimulation with IFNγ (10 ng/mL) and/or LPS (100 ng/mL) for 8 hours before the preparation of total RNA and analysis of the Nos2 mRNA level by northern hybridization. The data shown are representative of three independent experiments.
