Anti-Inflammatory Cytokine Interleukin-4 Inhibits Inducible Nitric Oxide Synthase Gene Expression in the Mouse Macrophage Cell Line RAW264.7 through the Repression of Octamer-Dependent Transcription
Figure 4
Mutational analysis of the mouse Nos2 enhancer/promoter region by luciferase reporter assays in RAW264.7 cells. The diagram on the left shows the wild-type (pNOS-996 and pNOS-333) and mutant Nos2 luciferase reporter constructs. Mutations of the NF-κB and/or OCT sites in the constructs are also indicated. RAW264.7 cells were transiently transfected with the wild-type or the mutant Nos2 luciferase reporter construct, as described above. The relative luciferase activities are shown as percentages of the activity in cells transfected with the wild-type construct (pNOS-996) and stimulated with IFNγ and LPS. Each column and bar represents the mean ± SEM of three independent experiments. The asterisks denote a statistically significant difference compared to the cultures treated with IL-4 (, Student’s test).
