The Mechanism of Sevoflurane Preconditioning-Induced Protections against Small Intestinal Ischemia Reperfusion Injury Is Independent of Mast Cell in Rats
Figure 3
Tryptase protein expression in intestine mucosa, and β-hexosaminidase levels in serum, intestinal mast cell counts after IIR injury. SH group (Sham-operated group), IIR group (75 min intestinal ischemia and 2 h reperfusion), IIR + CP group (IIR group + Compound 48/80 1 mg/kg), IIR + CS group (50 mg/kg cromolyn sodium treated IIR group), IIR + CS + CP group (50 mg/kg cromolyn sodium treated IIR group + Compound 48/80 1 mg/kg), SEV + IIR group (2.3% sevoflurane pretreated IIR group), and SEV + IIR + CP group (2.3% sevoflurane pretreated IIR group + Compound 48/80 1 mg/kg). Representative band of tryptase protein expression in intestinal mucosa is displayed in (a). Bar graphs (b) quantified tryptase protein expression (). Bar graph (c) quantified β-hexosaminidase levels (). Representative images of immunohistochemical staining for tryptase in intestine are displayed in (d) (SP staining, ×200); brown-yellow granules in the cytoplasm were recognized as mast cells. Bar graph in (e) quantified mast cell counts in small intestine (). Results are expressed as Mean ± SEM. versus SH group, versus IIR group, versus IIR + CP group, versus IIR + CS group, and versus SEV + IIR group.
