Intricacies for Posttranslational Tumor-Targeted Cytokine Gene Therapy
Figure 1
Expression and activity of IL12 is not affected by insertion of tumor-targeting sequence in either subunit. Multiple VNTANST-IL12 plasmids were created with the VNTANST sequence inserted directly prior to the stop codon in the p40 subunit (a), p35 subunit (b), or both subunits (c). The blue arrows represent the VNTANST-coding sequence insertion site in the plasmid DNA. CMV: cytomegalovirus promoter; IVS: intron; pA: bovine growth hormone polyadenylation signal; STOP: stop codon. (d) Transfection of these plasmids and wild-type IL12 and a control plasmid into cells resulted in equivalent amounts of expressed IL12 in the medium of transfected cells. (e) The peptide IL12 products induced equivalent levels of IFNγ from harvested murine splenocytes. # represents compared to all other groups.
