Research Article
Alliin, a Garlic (Allium sativum) Compound, Prevents LPS-Induced Inflammation in 3T3-L1 Adipocytes
Table 1
Primer sequences used for real-time quantitative PCR.
| | Gene name | Ensembl gene | Primer sequence (5′-3′) | bp | Tm |
| | Il-6 | ENSMUST00000026845 | F-TACACATGTTCTCTGGGAAATCGT
R-AAGTGCATCATCGTTGTTCATACA | 85 | 75 |
| | Aprt | ENSMUST00000006764 | F-CAGCGGCAAGATCGACTACA
R-AGCTAGGGAAGGGCCAAACA | 67 | 60 |
| | Mcp-1 | ENSMUST00000000193 | F-GCTGGAGAGCTACAAGAGGATCA
R-CTCTCTCTTGAGCTTGGTGACAAA | 79 | 60 |
| | Tnf- | ENSMUST00000025263 | F-ATGGCCCAGACCCTCACA
R-TTGCTACGACGTGGGCTACA | 73 | 60 |
| | Egr-1 | ENSMUST00000064795 | F-GCCGAGCGAACAACCCTAT
R-CCATCGCCTTCTCATTATTCAGA | 77 | 60 |
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