Heat Shock Proteins 60 and 70 Specific Proinflammatory and Cytotoxic Response of CD4⁺CD28^null Cells in Chronic Kidney Disease

Figure 3

Relative mRNA expression of IFN-𝛾, perforin, and granzyme B in CD4⁺CD28^null cells ((a), (c), (e)) as well as in CD4⁺CD28⁺ cells ((b), (d), (f)) in CKD subjects. 48 hr of stimulation of HSP60 (5 ng/mL) and HSP70 (5 ng/mL) increased the expression of IFN-𝛾, perforin, and granzyme B in both T-cell subsets compared to unstimulated controls. PHA (10 𝜇g/mL) served as a positive control for both T-cell subsets. **𝑃<0.0001, *𝑃<0.001.

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