Mediators of Inflammation / 2013 / Article / Fig 3

Research Article

Natural Antioxidant-Isoliquiritigenin Ameliorates Contractile Dysfunction of Hypoxic Cardiomyocytes via AMPK Signaling Pathway

Figure 3

ISL treatment stimulated cardiac AMP-activated protein kinase (AMPK) and ERK signaling pathways. Representative immunoblots of isolated mouse cardiomyocytes showed phosphorylation of (a) AMPK at Thr172 (p-AMPK), (b) ACC (Ser79), and (c) ERK. Phosphorylated AMPK was quantified relative to total AMPKα. Phosphorylated ACC and ERK were quantified relative to GAPDH. Values are expressed as means ± SE ( ), versus vehicle.