Leptomeningeal Cells Transduce Peripheral Macrophages Inflammatory Signal to Microglia in Reponse to Porphyromonas gingivalis LPS
Figure 1
Characterization of macrophages in gingiva of chronic periodontitis patients and cultured macrophages after P.g. LPS stimulation. Immunofluorescent CLMS images of TLR2, TLR4, TNF-α, and iNOS in gingiva of chronic periodontitis patients (a), scale bar = 20 μm. The mean mRNA levels of TNF-α, IL-10, iNOS, and Arg I of THP-1 human monocyte-like cell line after P.g. LPS (LPS, 1 μg/mL) treatment for 24 h (b). Data are presented by mean ± SEM (), *** versus nontreated cells (none). Time course of TNF-α and IL-1β release in RAW264.7 mouse macrophages after P.g. LPS treatment (c). Data are presented by mean ± SEM (, each), ***, * versus nontreated cells (none). The LPS-induced TNF-α secretion from RAW264.7 mouse macrophages at 48 h with the neutralizing antibodies against TLR2 (10 μg/mL), TLR4 (10 μg/mL), or a specific NF-κB inhibitor, Bay 11-7082 (Bay, 20 μM) (d). Data are presented by mean ± SEM (, each), *** versus nontreated cells (none), and ††† versus P.g. LPS alone.
