Leptomeningeal Cells Transduce Peripheral Macrophages Inflammatory Signal to Microglia in Reponse to Porphyromonas gingivalis LPS

Figure 2

Secretion of proinflammatory mediators by leptomeningeal cell after treatment with the conditioned medium from P.g. LPS-treated macrophages and P.g. LPS. The mean mRNA levels of TNF-α and IL-1β of leptomeningeal cells at 4 h incubated with P.g. LPS (LPS) and conditioned medium of P.g. LPS-treated RAW264.7 mouse macrophages (MCM) (a). Data are presented by mean ± SEM (𝑛=3), ***𝑃<0.001 versus nontreated cells (none). ^†††𝑃<0.001 versus P.g. LPS alone. Time course of TNF-α and IL-1β secreted by leptomeningeal cells after treatment with P.g. LPS (100 ng/mL) (b). Data are presented by mean ± SEM (𝑛=4, each), ***𝑃<0.001 versus nontreated cells (none). The P.g. LPS-induced TNF-α secretion by leptomeningeal cells at 6 h with the neutralizing antibodies against TLR2 (10 μg/mL), TLR4 (10 μg/mL), or Bay 11-7082 (Bay, 20 μM) (c). Data are presented by mean ± SEM (𝑛=4, each), ***𝑃<0.001 versus nontreated cells (none), and ^†††𝑃<0.001 versus P.g. LPS alone.