The Role of Mcl-1 in S. aureus-Induced Cytoprotection of Infected Macrophages
Figure 2
S. aureus increases both de novo Mcl-1 synthesis and stability. (a) Time course of MCL1 expression in control and S. aureus-infected macrophages (hours after-infection; p.i.) was monitored by qRT-PCR, as described in Section 2. Data represent the mean values calculated from the results of three independent experiments using hMDMs derived from different donors. Bars represent mean relative expression SD. ; . (b, c) Time course of Mcl-1 protein synthesis following S. aureus infection. Mcl-1 levels were measured at different time points between 0.5 and 20 h p.i. by immunoblot. (b) Representative immunoblot from three separate experiments performed on macrophages derived from different donors. (c) Relative Mcl-1 levels obtained by densitometric analyses of western blots. Results from three separate experiments. Data represent means SD. ; ; . (d) Mcl-1 stability in macrophages incubated in the presence of cycloheximide (CHX) (10 μg/mL) in the absence (circles) or presence (squares) of S. aureus (MOI 1 : 50). At time periods of up to 3 h, Mcl-1 levels were detected by immunoblot in cell lysates. Data represents Mcl-1 levels relative to time 0, which was arbitrarily set as 100%, obtained by densitometric analyses of western blots. Data represent means SD of three separate experiments. .
