The Role of Mcl-1 in S. aureus-Induced Cytoprotection of Infected Macrophages
Figure 6
NFκB is required for Mcl-1 expression induced by S. aureus. (a, b) hMDMs were pretreated for 30 min with Bay 11-7095 (40 μM) followed by S. aureus infection at an MOI of 50. After the indicated times, RNA and protein were extracted and Mcl-1 expression levels were determined by qRT-PCR and immunoblot (a and b, resp.). Data represent the means SD from three separate experiments. . (c) The effect of NFκB inhibition on IL-6 expression was measured by qRT-PCR. Shown are the mean values calculated from the results of five independent real-time reactions using hMDMs derived from different donors. Bars represent mean relative expression SD; .
