Ethyl Pyruvate Ameliorates Hepatic Ischemia-Reperfusion Injury by Inhibiting Intrinsic Pathway of Apoptosis and Autophagy
Figure 2
Effect of ethyl pyruvate on regulation of apoptosis. (a) The expression of Bcl-2 and Bax on cDNA level was detected by real-time PCR ( for saline VS saline + I/R, for saline + I/R VS I/R + EP (80 mg/kg). (b) The expression of Bcl-2 and Bax on protein level was detected by western blot. (c) Immunohistochemistry staining showed the expression of Bcl-2 and Bax protein in liver tissue at 8 h. Original magnifications: ×200. (d) TUNEL staining showed the apoptotic cells in three groups at 8 h. Original magnifications: ×100.