A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice
Figure 1
Schematic diagram depicting the experimental designs for this study. (a) Four treatment groups were used for in vivo experiments. At 1 and 6 hrs after NS (sham) or ZY injection, mice were subjected to inhalation of 0.7% ISO or RA (vehicle) for a 1 hr duration. BALF and lung tissues were harvested for a series of assays at 24 hrs following NS or ZY administration. (b) Test and control groups for 0.7% ISO treatment of isolated neutrophils. At 45 mins or 18 hrs after single or combined treatments with ZY, ISO or NF-κB activation inhibitor (NAI) in neutrophils, assays for NF-κB DNA-binding activity, iNOS activity, and NO formation were performed. DMSO was assayed as a solvent control. For more detailed descriptions, see Section 2.
