NF-B Inhibition after Cecal Ligation and Puncture Reduces Sepsis-Associated Lung Injury without Altering Bacterial Host Defense
Figure 1
Treatment with the IKK inhibitor (BMS-345541) inhibits NF-B activation after CLP. Nuclear protein extracts from (a) spleen and (b) lung tissue were probed for p65 (RelA) by western blot. Densitometry shows p65 band density normalized by TATA binding protein (TBP). ANOVA test was used for comparison among multiple groups. Tukey post hoc tests were undertaken after ANOVA. There were significant differences for CLP versus sham or CLP+BMS mice (* for CLP versus Ctrl, # for CLP+BMS versus CLP), while there was no significant difference between sham and CLP+BMS mice. (c) Lung NF-B DNA binding activity was also assessed in nuclear protein extracts by TransAM ELISA. ANOVA test was used for comparison among multiple groups. Tukey post hoc tests were undertaken after ANOVA. There were significant differences for CLP versus sham or CLP+BMS mice (* for CLP versus Ctrl, # for CLP+BMS versus CLP), while there was no significant difference between sham and CLP+BMS mice. Results are presented as mean ± SE, per group. Each experiment was repeated three times.
