High Sugar Intake and Development of Skeletal Muscle Insulin Resistance and Inflammation in Mice: A Protective Role for PPAR-δ Agonism

Figure 2

Effects of GW0742 treatment on insulin signal transduction in the gastrocnemius of mice fed with HFCS diet. Total IRS-1 protein expression and Ser³⁰⁷ phosphorylation (a), total Akt protein expression and Ser⁴⁷³ phosphorylation (b), and total GSK-3𝛽 protein expression and Ser⁹ phosphorylation (c) were analyzed by western blot on the gastrocnemius homogenates obtained from mice fed with a standard (control) or HFCS diet (HFCS) for 30 weeks and treated with GW0742 (1 mg/kg/day) added during the last 16 weeks (control+GW; HFCS+GW). Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the corresponding tubulin contents, and normalized using the related control band. The data are means±SD of three separate experiments. *𝑃<0.01 versus HFCS.

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