530429.fig.004a
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530429.fig.004b
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530429.fig.004c
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530429.fig.004d
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530429.fig.004e
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530429.fig.004f
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Figure 4: PA induces activation of IKKα and degradation of IκBα in HaCaT cells measured by western blotting. Keratinocytes were untreated or treated with PA (75, 100, 125, and 150 μmol/L) for 24 hours under serum-free conditions. The cells were harvested, and nuclear extracts were analyzed with IKKα by western blot immediately after the treatment (a) and 24 hours after the PA depletion (b). The densitometry values are means ± SD of three independent experiments (c). The protein expression of IκBα immediately after the treatment (d) and 24 hours after the PA depletion (e) were also determined by western blot. The densitometry values are means ± SD of three independent experiments (f). *indicate significant differences of , respectively, between the PA-treated groups and nontreated group immediately after the treatment groups (24 hours); #indicate significant differences of , respectively, between the PA-treated groups and nontreated group in 24 hours after the PA depletion groups (24 hours + 24 hours REC).