IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways

Figure 2

Effects of IL-21 on LPS-induced cytokine and chemokine mRNA expression in mouse peritoneal macrophages. Macrophages were incubated with IL-21 (100 ng/mL) in the presence or absence of LPS (100 ng/mL) for 3 h, 6 h, and 24 h. Total RNA was isolated and analyzed by real time RT- PCR for TNF-α (a), IL-6 (b), IL-1β (c), IL-10 (d), CCL5 (e), IFN-γ (f), and CXCL2 (g) mRNA expression. All of these expressions were normalized to endogenous control GAPDH. Results are expressed as mean ± SD for each group from three independent experiments. ^#𝑃<0.001 significantly different from the NT group; ^∗𝑃<0.05, ^∗∗𝑃<0.01 compared with the LPS group.

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