IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways

Figure 5

Effects of IL-21 on LPS-induced NF-κB signal pathway in mouse peritoneal macrophages. Macrophages (5×10⁶) were incubated with IL-21 (100 ng/mL) in the presence or absence of LPS (100 ng/mL) for 5 min, 20 min, and 60 min. Nuclear and cytosolic proteins from macrophages were analyzed by Western blot with specific antibodies. Detection of Actin and PCNA was estimated as protein-loading control for each lane. Values are presented as mean ± SD of three independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01 compared with the LPS group.

(a)

(b)

(c)

(d)