GM-CSF Exhibits Anti-Inflammatory Activity on Endothelial Cells Derived from Chronic Venous Disease Patients
Figure 2
GM-CSF treatment induces in vitro VEC proliferation. Dynamic monitoring of endothelial cells proliferation using the xCELLigence system. VEC were seeded at a density of 5.000 cells/well per well in quadruplicate in fibronectin-coated 16 wells E-plates and left adhere for 22 h when recombinant GM-CSF was added to the cultures at the indicated concentrations. In (a), cell proliferation was recorded and expressed as cell index (CI) after normalization to the CI recorded at the time of GM-CSF addition (arrow). A representative panel of GM-CSF-treated and untreated cell proliferation expressed as normalized CI is shown. In (b), the dose-dependent effect of GM-CSF in inducing VEC proliferation is shown as normalized CI of result from 4 independent VEC cultures. Horizontal bars are median, upper, and lower edges of box are 75th and 25th percentiles; lines extending from box are 10th and 90th percentiles. * compared to untreated VEC.
