CD38 Ligation in Peripheral Blood Mononuclear Cells of Myeloma Patients Induces Release of Protumorigenic IL-6 and Impaired Secretion of IFNγ Cytokines and Proliferation
Figure 1
Proliferation, IFNγ, and IL-6 induction by CD38 mAb engagement in PBMC obtained from MM patients or healthy individuals. PBMC (2 × 105 cells/well in 0.2 mL) obtained from MM patients or healthy controls were cultured in the presence of agonistic anti-CD38 IB4 (20 μg/mL). Proliferation (SI) was measured after 5 days by 3H-Thymidine incorporation. IFNγ (pg/mL) and IL-6 (pg/mL) were measured by ELISA after 18 hours of culture. (a) Dispersion of proliferation (SI) respect IFNγ (pg/mL) values in each of all PBMC donors tested. (b) Dispersion of proliferation (SI) respect to IL-6 (pg/mL) values in each of all PBMC donors tested. For the definition of SI and technical details, see text.
