CD38 Ligation in Peripheral Blood Mononuclear Cells of Myeloma Patients Induces Release of Protumorigenic IL-6 and Impaired Secretion of IFNγ Cytokines and Proliferation
Figure 2
IL-6 and PCNA induction by CD38 molecules engagement in PBMC obtained from MM, MGUS patients, or healthy individuals. (a) PBMC (1 × 106/mL) obtained from MM, MGUS patients, or healthy controls were cultured in the presence of agonistic anti-CD38, anti-CD3, or unstimulated (none). Taqman Real Time quantitative RT-PCR for IL-6 gene expression was performed at 18 hours time-point. mRNA transcript levels were expressed as fold increase over the unstimulated PBMC. (b) PBMC (1 × 106/mL) obtained from MGUS, MM patients, or healthy individuals were cultured in the presence of agonistic anti-CD38, anti-CD3, or unstimulated (none). Taqman Real Time quantitative RT-PCR for PCNA gene expression was performed at 18 hours time-point. mRNA transcript levels were expressed as fold increase over the unstimulated PBMC. For further technical details, see text.
