Figure 3: Leukocyte is ruptured by long-term filtration. Groups were the same as in Figure 1 ( in each group). (a) Transmission electron microscope of leukocyte. Cellular membrane rupture and cytoplasmic leakage (yellow arrow) were found in Group L. (b) Wright’s stain of nucleated cell. (c) Percentage of naked leukocyte nuclei were higher in Group L than these in groups C and S. ** versus Group C; ## versus Group L.