Modulation of Conjunctival Goblet Cell Function by Inflammatory Cytokines
Figure 6
Inflammatory cytokines alter proliferation and apoptosis rate of cultured conjunctival goblet cells. Cultured goblet cells were pulsed with BrdU and treated for 24 h with 10 ng/mL of IL-13, IFN-γ, TNF-α, IL-6, or IL-17A. Cells stained with fluorescence-conjugated anti-BrdU antibody and viability dye 7-AAD was analyzed by flow cytometry to identify stages of cell cycles. (a) Representative flow cytometry plots are shown and percentage of cells in each of the G0-G1, S + G2-M phases and apoptotic cells are indicated for untreated control and IL-13 treated cells. (b) Bar graphs show changes in proliferation (S + G2-M phases) and apoptosis relative to untreated controls.
