Mediators of Inflammation / 2013 / Article / Fig 5

Research Article

The Role of EGFR/PI3K/Akt/cyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma

Figure 5

EGFR/PI3K/Akt signaling pathway is required for cyclinD1 upregulation in EGF-stimulated EACKs. Cells were treated with 15 ng/mL of EGF for the indicated periods of time ((a), (b), (e)) or incubated with or without AG1478 or wortmannin for 1 h and then exposed to 15 ng/mL of EGF for the indicated periods of time ((c), (d), (f)). After treatment, cells were harvested for Western blotting analysis using specific antibodies as indicated, and β-actin was used as an equal loading control.
651207.fig.005a
(a)
651207.fig.005b
(b)
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(c)
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(d)
651207.fig.005e
(e)
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(f)

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