Local Overexpression of Interleukin-11 in the Central Nervous System Limits Demyelination and Enhances Remyelination
Figure 3
IL-11 inhibits myelin phagocytosis in vitro. BV2 cells were pretreated with increasing amounts of rmIL-11 for 12 h. Medium only was used as control. Subsequently cells were allowed to phagocytose DiI-labeled myelin for 90 min. (a) A representative single-parameter histogram showing IL-11-induced dose-dependent inhibition of DiI-labelled myelin phagocytosis. (b) Bar graph showing mean fluorescence intensity of DiI. The experiment was repeated three times and was performed in triplicate each time. Data is presented as mean ± SEM of triplicate determinations from three independent experiments. Statistical significance is analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison test. *** as compared with medium only group.
