Flavonoids and 5-Aminosalicylic Acid Inhibit the Formation of Neutrophil Extracellular Traps
Figure 2
Influence of antioxidants on neutrophil ROS formation as measured by the luminol assay. Freshly isolated neutrophils were preincubated with or without antioxidants for 30 min at 37°C. Following addition of luminol (0.06 mM) and induction by 20 nM PMA, the kinetic of ROS formation was measured over a period of 1 h at 37°C. ROS formation of (a) epicatechin, (b) catechin hydrate, (c) rutin trihydrate, (d) ascorbic acid and tocopherol, (e) melatonin, (f) N-acetyl-L-cysteine (NAC), (g) urea and acetylsalicylic acid (ASS), and (h) 5-aminosalicylic acid (5-ASA) was quantified by calculation of the area under the curve (AUC). Data were normalized to the sample with PMA-stimulated neutrophils without antioxidants (Med.). Data show mean SD from 3 ((d), (f), (g) (urea), (h)), 5 ((a), (c), (e), (g) (ASS)) or 7 (b) independent experiments with 2 ((g), ASS), 3 ((a), (d), (e), (f), (g) (urea)), 4 ((b), (c)) and 5 (h) different donors. ; ; as compared to the PMA-stimulated medium or solvent control (Med., Solv., black column).
