Flavonoids and 5-Aminosalicylic Acid Inhibit the Formation of Neutrophil Extracellular Traps
Figure 3
Influence of antioxidants on extracellular ROS formation of neutrophils as measured by the lucigenin assay. Neutrophils were preincubated with or without antioxidants for 30 min at 37°C. Following addition of lucigenin (0.2 mM), ROS formation was induced by 20 nM PMA and the kinetic of -formation was measured over a period of 1 h at 37°C. -formation of (a) epicatechin, (b) catechin hydrate, (c) rutin trihydrate, (d) ascorbic acid and tocopherol, (e) melatonin, (f) N-acetyl-L-cytseine (NAC), (g) urea and actetylsalicylic acid (ASS), and (h) 5-aminosalicylic acid (5-ASA) was quantified by calculation of the area under the curve (AUC). Data were normalized to the sample of PMA-stimulated neutrophils without antioxidants (Med.). Data show mean SD from 3 ((d), (f), (g) (urea)), 5 ((a), (e), (g) (ASS), (h)), 6 (c) or 7 (b) independent experiments with 2 ((g), ASS), 3 ( (a), (d), (e), (f), (g) (urea)), 4 (b) and 5 ((c), (h)) different donors. ; ; as compared to the PMA-stimulated medium or solvent control (Med., Solv., black column).
