Flavonoids and 5-Aminosalicylic Acid Inhibit the Formation of Neutrophil Extracellular Traps

Figure 6

Influence of antioxidants on the formation of NETs as assessed by fluorescence and scanning electron microscopy. Freshly isolated neutrophils were pre-incubated with or without antioxidants (0.5 mM 5-aminosalicylic acid, 10 mM N-acetylcysteine, 2 mM ascorbic acid, 0.1 mM epicatechin, 0.1 mM catechin hydrate, 0.075 mM rutin trihydrate) for 30 min at 37°C. The DNA was stained with 100 nM SYTOXgreen for detection with fluorescence microscopy (FM). Afterwards, NET formation was induced by stimulation with PMA (20 nM scanning electron microscopy (SEM), 10 nM FM). The samples were fixed after 4 h (SEM) and 3 h (FM). Scale bars represent 5 μm. Neutrophils stimulated with PMA (Medium) and coincubated with DMSO (solvent control) were used as controls.