Mediators of Inflammation / 2013 / Article / Fig 1

Research Article

Hmgb1-IL-23-IL-17-IL-6-Stat3 Axis Promotes Tumor Growth in Murine Models of Melanoma

Figure 1

A defect in IL-17 inhibits tumor development. Mice were inoculated s.c. with tumor cells, and tumor sizes were monitored. (a) Wild-type mice and IL-17−/− mice were inoculated with B16-F10 tumor cells ( ). (b) Wild-type mice were treated i.v. with Ad-IL-17 or Ad-GFP (109 PFU/mouse) or left untreated (none). Two days later, the mice were inoculated with B16-F10 tumor cells, and tumor growth was monitored ( ). (c) Wild-type mice were inoculated with B16-F10 tumor cells and injected i.p. with normal rat-IgG or a rat anti-mouse IL-17 mAb (100 μg/mouse) on days 0, 1, 6, 10, and 14 ( ). Control mice were left untreated (none). (d) CD11b+Gr-1+ MDSCs in the spleen, blood, and tumor samples from tumor-bearing mice inoculated with B16-F10 tumor cell 2 weeks ago were analyzed by FACS ( ). (e) MDSCs were purified from spleens of B16 tumor-bearing mice and stimulated overnight with LPS. CD11b+ cells from naïve tumor-free mice were treated with LPS and served as controls. mRNA levels were determined by realtime RT-PCR and normalized to -actin in each sample ( ). The data show means ± SEM of tumor size and are representative of three independent experiments. .
713859.fig.001a
(a)
713859.fig.001b
(b)
713859.fig.001c
(c)
713859.fig.001d
(d)
713859.fig.001e
(e)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.