Hmgb1-IL-23-IL-17-IL-6-Stat3 Axis Promotes Tumor Growth in Murine Models of Melanoma
Figure 6
IL-17 activates Stat3 in tumor dependent on IL-6 production. (a) IL-6 level in B16 tumor cells treated for 24 hours with recombinant IL-17 was assessed by quantitative realtime PCR. (b) IL-6 level in B16 tumor cells treated for 24 hours with recombinant IL-17 was assessed by ELISA. (c) Signal-cell suspensions prepared from B16 tumors harvested from WT, WT + anti-γ δ TCR, and IL-17−/− groups were cultured in vitro overnight; supernatants were then collected and assayed for IL-6 levels (). (d) Tumor growth in B16 tumor cells inoculated mice from WT + Rat-IgG, WT + anti-IL-6, WT + anti-IL-6 + Ad-IL-17, IL-17−/− + Ad-GFP, and IL-17−/− + Ad-IL-6 groups (). (e) Indicating p-Stat3 protein levels (analyzed by Western blot) in B16 tumor tissues from WT + Rat-IgG, WT + anti-IL-6, WT + anti-IL-6 + Ad-IL-17, IL-17−/− + Ad-GFP, and IL-17−/− + Ad-IL-6 groups. The data show means ± SEM of tumor size and are representative of three independent experiments. . NS = not significant.
