Mediators of Inflammation

Research Article

Effects of ω-3 Polyunsaturated Fatty Acids on Plasma Proteome in Rett Syndrome

Table 1

Identification results of proteins differentially expressed in basal and in RTT before and after ω-3 PUFAs supplementation.
ExperimentalData bank exploration
SpotProtein nameUntreated RTTRTT after ω-3 supplementationUniProt IDEntry nameMascot scoreSequence coverage (%)Peptides matchesTheoretical pI/Mr (kDa)
ANOVA (P < 0.05)Fold change*Protein expressionANOVA (P < 0.05)Fold change**Protein expression
1Complement Factor B0.042+1.58Upregulated0.030−1.40DownregulatedP00751CFAB1022111/246.67/86.4
2Fibrinogen alpha chain0.009+2.07Upregulated0.008−2.04DownregulatedP02671FIBA1203025/865.70/95.6
3Albumin0.016+1.67Upregulated0.012−1.66DownregulatedP02768ALBU2423822/335.92/71.3
4Alpha-1-antitrypsin0.001+1.97Upregulated0.019−1.33DownregulatedP01009A1AT2356026/695.37/46.8
5Alpha-1-antitrypsin0.003+1.73Upregulated0.005−1.47DownregulatedP01009A1AT1062510/185.37/46.8
6Vitamin D-binding protein0.002−1.52Downregulated0.001+1.85UpregulatedP02774VTDB1233514/405.40/54.5
7Albumin (C terminal fragment)0.027+1.46Upregulated———P02768ALBU79189/255.92/71.3
8Apolipoprotein A-IV0.038−1.45Downregulated0.029+1.46UpregulatedP06727APOA41073613/485.28/45.3
9Haptoglobin0.010+1.17Upregulated0.027−1.15DownregulatedP00738HPT58169/236.13/45.8
10Clusterin0.013−1.34Downregulated0.009+1.50UpregulatedP10909CLUS131248/85.89/53.0
11Transthyretin0.012+1.91Upregulated0.015−1.79DownregulatedP02766TTHY113596/105.52/15.9
12Apolipoprotein A-I0.043−1.50Downregulated0.024+1.52UpregulatedP02647APOA12537027/695.56/30.7
13Retinol-binding protein 40.001−2.56Downregulated0.001+2.59UpregulatedP02753RET4112468/185.76/23.3
14Albumin (N terminal fragment)0.021+1.73Upregulated0.014−1.78DownregulatedP02768ALBU1432414/235.92/71.3
15Haptoglobin0.001+1.81Upregulated0.003−1.55DownregulatedP00738HPT722110/316.13/45.8
16Transthyretin0.044−1.32Downregulated———P02766TTHY120687/155.52/15.9
Experimental section includes Spot ID, protein name, and the significant threshold level (P < 0.05). Variation in spot intensities represented by fold is expressed as ratio of RTT to control and as a ratio of RTT after ω-3 supplementation to RTT. Positive fold changes denote proteins upregulated, while negative fold changes denote decrease of protein expression levels. — denotes the absence of significant changes. Data bank exploration of proteins includes SwissProt data bank accession numbers and entry name, the Mascot score, the sequence coverage, the number of peptides matching the protein sequence, and theoretical pI/Mr × 10−3. *Comparisons versus healthy controls; **Comparisons versus untreated RTT.