Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice

Figure 1

CB2R protects against LPS-induced shock and proinflammatory cytokines production in mice. (a) CB2R^+/+ (𝑛=15) and CB2R^−/− (𝑛=15) mice were challenged with LPS (15 mg/kg, i.p.). Survival was assessed for 24 h. **𝑃<0.01. (b)–(d), ELISA detection of serum TNF-𝛼, IL-6 and HMGB1 levels 3 h after LPS (5 mg/kg) challenge in CB2^+/+ (𝑛=5) or CB2^−/− (𝑛=5) mice. Data are means ± SEM *𝑃<0.05, and **𝑃<0.01. (e) Observation of 72 h survival rate in C57BL/6 mice treated with LPS (30 mg/kg, i.p.) in the absence of presence of indicated doses of GW405833 (i.p.). Mice treated with vehicle as control. 𝑛=15 per group and *𝑃<0.05 versus vehicle. (f)–(h) ELISA detection of serum TNF-𝛼, IL-6, and HMGB1 levels 6 h after LPS (5 mg/kg, i.p.) challenge in the absence or presence of indicated doses of GW405833 (i.p.). 𝑛=8 in vehicle, 𝑛=5 in 3 mg/kg, 𝑛=10 in 10 mg/kg, data are means ± SEM, and **𝑃<0.01 versus vehicle.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)